Stimulation of Osteoconductivity by Ultraviolet Irradiation on Titanium Surfaces in Senile Osteoporotic Model Mice in in vivo and in vitro

Abstract

The effect of UV-photofunctionalization on the osteoconductivity of titanium implanted under osteoporotic conditions using the SAM-P/6 senile osteoporosis mouse model was evaluated. UV-photofunctionalized titanium was prepared by treatment of the aged titanium surfaces with UV irradiation. Titanium implants were fixed and subjected to push out testing. The morphology of new bone around the titanium plate was evaluated. Cell quantity, ALP activity and calcification of each titanium disc ware determined. The UV-photofunctionalized implants showed significantly higher maximum push out value (N) compared with the aged-surface controls. Bone contact ratio, cell quantity, ALP activity and calcification ware significantly higher for the UV-photofunctionalized titanium compared with the aged-surface controls. We established a system to study implant placement in this senile osteoporosis model, and conducted biomechanical, histological, and cytological investigations. Based on these investigations, we propose that UV-photofunctionalization enhanced osteoconductivity in osteoporotic conditions in the SAM-P/6 model of senile osteoporosis used in this study.