Examination of an Evaluation Method for Health Effects of Exposure to Gas and Particulate Matter Under Air-Liquid Interface Culture Conditions

-Gene Expression Analysis of Airway Epithelial Cells Exposed to Cigarette Smoke-

Abstract

To examine the usefulness of our cell exposure system for the evaluation of inflammatory responses, we analyzed the expression of inflammation-related genes in airway epithelial cells exposed to cigarette smoke using the cell exposure system. As a result, the expression change of inflammation-related genes induced by cigarette smoke could be observed in adenocarcinomic human alveolar basal epithelial cells (A549) and human airway epithelia (MucilAirTM). This result shows the usefulness of our cell exposure system for the evaluation of inflammatory responses.