リポ蛋白質の過酸化変性について

抄録

Peroxide degeneration of lipoproteins was studied on twenty-seven patients with hyperlipidemia and cerebral infarction. After adding peroxide-generating substances to the serum and incubating it at 37°C, the extent of lipoprotein degeneration was measured immediately and 30, 60, 120, 180 minutes after adding respectively with the use of agarose-gel electrophoresis. In addition SH groups, LPO and Vitamin E were measured simultaneously.
The results are as follows:
1) No change in lipoprotein pattern was recorded when Arachidonic acid (1mg/ml) was added, but change was detected when 15HPAA (1mg/ml) or H₂O₂ (3%) was added.
2) After H₂O₂ was added to the serum, the β peak shifted to the right and the pre-β peak shifted to the left, and the α peak became smaller accompanying structural collapse with bimodial distribution and these changes were detected earlier in the diseased group than in the healthy group.
3) When the difference between the 30 minute value and the 0 minute value of the ratio of the heights of the β and α peaks was used as the index to calculate the above changes, the healthy group showed 0.41±0.08, the hyperlipidemia group, 1.0±0.67, and the cerebral infarction group, 0.83±0.55. These values indicated that the extent of peroxide degeneration of lipoprotein was significant in the diseased group, where only a short time was required for its appearance.
4) With regard to the biochemical changes occurring at the same time, the SH groups recorded a 60% drop immediately after H₂O₂ was added, LPO showed a 15% rise, followed by a gradual increase, and V. E indicated a mild decline.
5) The inhibitory effect of V. E on lipoprotein degeneration was detected after administering 1, 200mg/day of V. E to cerebral infarction patients for weeks.