Abstract
It is well known that pyridoxal phosphate (PAL-P) works as a cofactor of lysyl oxidase, an enzyme that is absolutely necessary to form the crosslinking in collagen and elastin and β-aminopropionitrile (BAPN) produces dissecting aneurysm in aorta of rats and another animals, because it inhibits the activity of lysyl oxidase. But the direct relationship between PAL-P and BAPN is unknown, thus authors studied physiological effects of PAL-P on enzymatic reactions of cross-linking formation.
In this study, authors observed that PAL-P combined with BAPN and considerably inhibited the depression of activities of vitamin B6 dependent enzymes by BAPN in vitro. In addition, allylamine and cysteamine that have been reported as toxic agents for vascular wall, also inhibited the activities of vitamin B6 dependent enzymes as same as BAPN and INH.
From the above results, it was assumed that BAPN inhibited the lysyl oxidase by binding with PAL-P that is necessary for the activation of lysyl oxidase.
