Effect of Age on Plasma Lipoprotein Metabolism in Nonobese Diabetic Men: Analysis of Cholesterol and Protein in Apoprotein A-I or B-100 Containing Particles Fractionated by Affinity Columns with Monoclonal Antibodies against Apoprotein A-I or B-100

Abstract

Apoprotein A-I and B-100 containing particles were isolated by selective affinity columns prepared with monoclonal antibodies. The effect of age on the cholesterol and protein concentrations in these particles were studied in 56 noninsulin-dependent diabetic men and 59 nondiabetic men. Neither group was obese. In both groups, the plasma cholesterol values were less than 230mg/dl, while the plasma triglyceride values were less than 150mg/dl. The total cholesterol (Ch), free cholesterol (FC), cholesterol ester (CE), the ratio of CE/FC, protein content, and the ratio of Ch/protein content in apoprotein A-I and B-100-containing particles in each group were not significant between the 4 age groups.