Effect of Ethyl Icosapentaenoate (EPA) on the Concentration of Tumor Necrosis Factor (TNF) and Interleukin-1 (IL-1) in the Carotid Artery of Cuffsheathed Rabbit Models

抄録

To evaluate the effect of ethyl icosapentaenoate (EPA) on the metabolism of tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β), the concentrations of these cytokines in the carotids of rabbits sheathed in a cuff were studied. Japanese white rabbits were divided into two groups ; the EPA group, in which 600 mg/kg/day EPA was administered forcibly p.o. for 1 week before cuff treatment, and the control group. Carotid artery samples were obtained just before, 3 days and 7 days after cuff treatment, and TNFα and IL-1β were determined separately with the Western blot analysis method. In the control group, there were 43.5 (±3.0) pg/rig protein of TNFα and 53.5 (±4.8) pg/μg protein of IL-1/β just before cuff treatment. Compared to the control group, these concentrations of the EPA group were both significantly low. Three days after cuff treatment, TNFα of the EPA group was still significantly low, while IL-1β showed no difference. There was no significant difference between the two groups 7 days after cuff treatment. These findings suggested that EPA could influence TNFα and IL-1β metabolism in the arterial wall even at baseline. Furthermore, EPA suppressed TNFα and IL-1β production in the early phase of intimal thickening, indicating a mechanism inhibiting the activation of smooth muscle cells such as their proliferation and migration, induced by the cuff-sheath method. J Atheroscler Thromb, 2001; 8 45-49.