Abstract
The effects of ipriflavone (TC-80) administered with or without 1α, 25-dihydroxycholecalciferol (DHCC) on morphological and mineral changes in the calvarium have been examined in neonatal rats. DHCC (5-20ng/day/rat), TC-80 (62.5-125μg/day/rat), or both were injected subcutaneously once a day for five days. The morphological changes were evaluated by microensitometry of soft x-ray photographs of the calvarium, and the mineral changes were evaluated by chemical and x-ray powder diffraction methods.
DHCC-treated group had smaller-sized calvaria and wider sutures compared to control gorup as measured by soft x-ray photography. The mineral contents of the asked calvarium, Ca/P ratios in the ash, and x-ray diffraction variables which correspond to hydroxyapatite contents in the ash, were also reduced by DHCC. The addition of TC-80 tended to normalize these variables. The results show that when DHCC is administered to rat pups in the doses used, it demineralizes the calvarium in a dose-dependent manner; the simultaneous administration of TC-80 inhibits the bone changes induced by DHCC.
