Selective production of cellulase and hemicellulase from Aspergillus oryzae using starch-free wheat bran.

Abstract

In order to produce enzymes for degrading hemicellulose--which is the main component of wheat bran (WB), starch-free wheat bran (SFWB) was prepared by acid hydrolysis in an autoclave and cleaning with hot water. After culturing Aspergillus oryzae for 7 days with SFWB as a substrate, the expression of α-amylase was suppressed, while the production of hemicellulase and cellulase was confirmed; these were not observed in the WB culture. In addition, the enzyme activity of β-xylanase and β-xylosidase was increased. Enzyme production from wheat bran hemicellulose-derived hemicellulase is now feasible using Aspergillus cultured in SFWB.