2021 Volume 18 Issue 1 Pages 7-21
Objective: Placental extracts (PEs) have been used for years as a folk remedy in Asian countries. PEs mediates alleviation of menopausal symptoms, wound healing and liver regeneration. In this study, we evaluated the effect of porcine placental extract (PPE) on monocyte differentiation into dendritic cells (DCs) and cytokine production by monocytes following stimulation with PPE.
Methods: Human myelomonocytic cell line, THP-1 were treated with PPE or human placental extract (HPE) in the presence or absence of lipopolysaccharide (LPS) and interferon (IFN)-γ, then the secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-6 was measured by specific ELISA and real-time PCR. We also evaluated the effect of PPE on the expression of the monocytic markers, CD11b, CD80 and HLA-DR, on cultured THP-1 cells as well as DCs derived from peripheral blood mononuclear cells (PBMC) in postmenopausal patients after oral administration of PPE.
Results: PPE or HPE significantly decreased to normal levels the enhanced secretion of IL-6 and TNF-α by THP-1 cells, in the presence of LPS. However, both PPE and HPE enhanced the production of these cytokines upon stimulation of the cells with IFN-γ. PPE also increased the expression of CD11b and CD80 in cultured THP-1 cells stimulated with IFN-γ. Further, climacteric healthy women treated orally with PPE three times daily for a month showed an increase in surface makers for dendritic cells in PBMC.
Conclusion: We found scientific evidence of an immune modulation by PPE treatment. It is therefore presumed that PPE might represent an immunomodulating adjuvant for immune diseases.