JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Online ISSN : 1881-1299
Print ISSN : 0021-9592
Biochemical, Food and Medical Engineering
Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum: Use as an Animal Cell Culture Substratum
Hiroyuki IjimaMotonori NakashimaSachiko FurutaTsutomu OnoKoei Kawakami
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2004 Volume 37 Issue 4 Pages 531-535

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Abstract

A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150–200 μm, and a porosity of 28% that was dependent on the PMMA beads quolity. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·ml–1-HAp. This value is similar to most existing suitable porous microcarriers.
A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

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© 2004 The Society of Chemical Engineers, Japan
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