Journal of the Ceramic Society of Japan
Online ISSN : 1348-6535
Print ISSN : 1882-0743
ISSN-L : 1348-6535
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Enhancement of cell differentiation on a surface potential-controlled nitrogen-doped TiO2 surface
Masami HASHIMOTOSatoshi KITAOKAMaiko FURUYAHiroyasu KANETAKAKazuhiko HOSHIKAYAHayato YAMASHITAMasayuki ABE
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2019 Volume 127 Issue 9 Pages 636-641

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Abstract

MC3T3-E1 cell differentiation is more pronounced after 14 days incubation on charged nitrogen-doped TiO2 surfaces compared to on an untreated, neutral Ti surface. The protein fibronectin (Fn) was detected by an immunogold-labeling technique and Ca and P were detected by time-of-flight secondary ion mass spectrometry. Both techniques revealed that an adhesive protein such as Fn adsorbs equally on negatively-charged, positively-charged, and untreated Ti surfaces in culture medium. However, the adsorption of Ca and P was only detected on charged nitrogen-doped TiO2 surfaces. The enhanced adsorption of inorganic ions and Fn is probably responsible for promoting initial stage of osteoblast differentiation. The conformation of adsorbed Fn was observed by high-speed atomic force microscopy and found to be in the side-on orientation on the positively-charged surface. This finding may help elucidate the relation between Fn conformation and cell activity on surface potential-controlled nitrogen-doped TiO2 surfaces in future.

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© 2019 The Ceramic Society of Japan
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