2021 Volume 66 Pages 7-11
A thiol-specific oxidant diamide induces the generation of reactive oxygen species and the peroxidation of lipids. In this study, we developed the method to evaluate diamide sensitivity in rice. Our method revealed that there are genetic differences in the diamide sensitivity among rice cultivars. Shoot and root growth of all cultivars tested was remarkably inhibited at the concentration of 2 mM diamide for 7 days treatment. Among cultivars tested, a japonica cultivar Koshihikari showed high tolerance to diamide, and an indica cultivar Nanjing 11 showed a large decrease of root elongation. In two japonica cultivars Akichikara and Hounen-Wase, there was no significant difference in growth inhibition between shoot and root. Furthermore, by using our method, we successfully isolated diamide-sensitive and -tolerant mutants among 328 M2 lines derived from gamma-irradiated seeds of a japonica cultivar Gimbozu.