日本結晶学会誌
Online ISSN : 1884-5576
Print ISSN : 0369-4585
ISSN-L : 0369-4585
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超分子複合体の構造解析における諸問題とその解決法
今崎 剛高木 雄一郎
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2012 年 54 巻 5 号 p. 292-299

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Deciphering structures of the multi-protein complexes is vital to understand biology. However, the large size, complexity, heterogeneity, and typically low abundance in their native sources, have often precluded successful structural analysis of the multi-protein machines. We have determined the crystal structure of the Mediator Head module (7 subunits, 223 kDa), an essential component in RNA polymerase II transcription. Our success depended on addressing series of challenges unique to structure determination of multi-protein complexes. We anticipate that our approach will serve as a paradigm for structural analysis of the eukaryotic multi-protein complexes that regulate vital cellular processes in the future.

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© 2012 日本結晶学会
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