最近の研究から
湿度調整と水溶性ポリマーのコーティングを用いたタンパク質結晶マウント法
2014 年 56 巻 3 号 p. 194-200
詳細
2014 年 56 巻 3 号 p. 194-200
A novel protein crystal mounting method was developed using controlled humid air and hydrophobic polymer glue for crystal coating. The glue-coated crystals under the optimized humid air were quite stable at room temperature and could be cryocooled. This method is particularly useful when applied to fragile protein crystals known to be sensitive to environmental change. And, the crystals by using HAG method reproducibly showed crystal lattice transformation in response to a change in humidity, thus using this method a series of isomorphic crystals can be prepared.
