We report a method of femtosecond crystallography for determining radiation damage-free crystal structures of large proteins at atomic resolution. The name of the method is ʻserial femtosecond rotation crystallographyʼ (SF-ROX). Here, we demonstrate experimental details of SF-ROX and 1.9-Å radiation damage-free structure analysis of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
