日本結晶学会誌
Online ISSN : 1884-5576
Print ISSN : 0369-4585
ISSN-L : 0369-4585
総合報告
Cas9の結晶構造
西増 弘志
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ジャーナル フリー

2015 年 57 巻 2 号 p. 96-103

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Cas9 is an RNA-guided DNA endonuclease and is implicated in the bacterial CRISPR-Cas adaptive immune system. Cas9 binds dual guide RNAs or single-guide RNA, and the Cas9-RNA complex recognizes and cleaves target double-stranded DNA via RNA-DNA complementarity. To understand this unprecedented, RNA-guided DNA cleavage mechanism, we solved the crystal structure of Cas9 in complex with guide RNA and target DNA. The ternary complex structure provides insights into the RNA-guided DNA cleavage mechanism, and paves the way for the rational design of new, Cas9-based tools.

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© 2015 日本結晶学会
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