The ring-shaped cohesin complex entraps chromosomes and regulates chromosome biology. The cohesin core consists of Smc1, Smc3, Scc1 and Scc3. Scc2-Scc4 complex is known as a cohesin loader and loads cohesin onto chromosomes. Mutations of Scc2 cause human developmental diseases termed cohesinopathy. Here we determined the crystal structure of Chaetomium thermophilum（Ct）Scc2 and its interaction with cohesin. The structure consists mostly of HEAT repeats and it forms a hook-shaped structure that similar with Scc3 and Pds5. Many cohesinopathy mutations in Scc2 diminish the Scc2- Scc1 interaction. Our study defines a functionally important interaction between cohesin and Scc2.
編集・発行 : 日本結晶学会 制作・登載者 : 日本印刷株式会社