日本結晶学会誌
Online ISSN : 1884-5576
Print ISSN : 0369-4585
解説
タンパク質の電子線三次元結晶構造解析と電荷の精密化
米倉 功治眞木 さおり
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ジャーナル フリー

59 巻 (2017) 2-3 号 p. 88-95

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Electron 3D crystallography is a useful method for structure analysis from tiny and thin crystals of membrane proteins and protein complexes, which often yield crystals too small or too thin for even the synchrotron X-ray beam and X-ray free electron laser. More importantly, it can visualize the charged states of amino-acid residues and metals, as the diffraction pattern formed by elastically scattered electrons is directly related to the distribution of Coulomb potential. Here we introduce the development of this technique and structure determination with charges, and discuss further applications including a suitable treatment of electron scattering factors of charged atoms.

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© 2017 日本結晶学会
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