Deducing Free-Energy Landscape of Biomolecular Dynamics from Cryo-Electron Microscopy Images

Abstract

Cryo-electron microscopy（cryo-EM）is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble of the target biomolecule, cryo-EM is applicable to analyze the biomolecular dynamics such as metastable conformations and/or free-energy landscape in conformational changes. Here, we briefly introduce our method to deduce free-energy landscape of biomolecules from cryo-EM images and compare it with other existing methods.