日本結晶学会誌
Online ISSN : 1884-5576
Print ISSN : 0369-4585
ISSN-L : 0369-4585
膜融合を利用したバクテリオロドプシンの結晶化法
竹田 一旗神山 勉
著者情報
ジャーナル フリー

2000 年 42 巻 2 号 p. 171-175

詳細
抄録

Bacteriorhodopsin, the sole protein in the purple membrane of Halobacterium salinarium, functions as a light-driven proton pump. To obtain it's three-dimensional crystal, we have developed a new crystallization method which does not involve any step of complete destruction of the membrane. First, purple membrane was incubated at high temperature with neutral detergent and ammonium sulfate so that the membrane was converted into uniformly sized spherical vesicles. Then the vesicles were condensed at low temperature, where fusion of the vesicles produced a hexagonal crystal which is made up of planar membranes. Structural analysis showed that the trimeric structure of bacteriorhodopsin was retained in the crystal. The native lipid bound to a specific site in the protein was suggested to play an important role in maintaining the higher-order structure of the membrane.

著者関連情報
© 日本結晶学会
前の記事 次の記事
feedback
Top