2000 年 42 巻 4 号 p. 361-366
The three-dimensional structure of catechol 2, 3-dioxygenases (metapyrocatechase, MPC), which catalyzes the proximal extradiol cleavage reaction of catechol and 3- or 4-substituted catechol, has been determined at 2.8Å resolution. The enzyme is a homotetramer with non-crystallographic 222 symmetry and each subunit is folded into two similar domains. The active site structure reveals a distorted tetrahedral Fe (II) site coordinated by three endogenous ligands (His 153, His214, and G1u265) and an acetone molecule.