The crystallographic phase problem had not been routinely solved in protein crystallography. Nowadays, MAD method is widely used for crystallographic determination of unknown protein structures, since the scope of MAD application has been extended by the use of synchrotron radiation facilities, protein expression techniques, and computational development. The method has paved the way to high-throughput structure determination of protein crystals and introduced to structural genomics study. We describe here a brief summary of MAD method and its experimental.
