2008 年 50 巻 2 号 p. 138-143
1, 2-α-L-Fucosidase (AfcA) is an exoglycosidase recently identified from Bifidobacterium bifidum. To elucidate the molecular mechanism of this enzyme, we determined the X-ray crystal structures of the AfcA catalytic domain in unliganded and complexed forms with inhibitor, substrate and products at atomic resolution. A series of the structural and the biochemical analyses provides insight into a novel catalytic mechanism for this enzyme.