Investigations were carried out to determine the effects of culture conditions of lowland and upland with intermittent irrigation after nearly young panicle formation stage on lipid content and fatty acid composition of lowland brown rice of non-glutinous and glutinous varieties. The difference between lowland and upland cultures was not significant in the lipid content. On the other hand, five fatty acids except myristic acid, palmitic acid, and arachidic acid were affected by the culture condition in the content. The lowland culture, as compared with the upland culture, gave significantly high oleic acid content and low stearic acid and linoleic acid contcnt of both non-glutinous and glutinous samples. As to non-glutinous rice and glutinous rice, the latter was significantly higher in lipid content and in palmitic acid content by both the cultures and in myristic acid content by lowland culture and lower in arachidic acid content by lowland culture.