Determination of the three-dimensional structures of proteins is one of the key postgenomic research subjects. X-ray crystallography and solution NMR are both used for structural determination; the present paper reviews the process of protein structural determination by NMR. The NMR facility of RIKEN Genomic Sciences Center, constructed in Yokohama in 2000, and the “structural genomics project” being pursued using the NMR facility are briefly described.