Abstract
A high performance liquid chromatographic analysis for the simultaneous determination of ammonia, nitrite, nitrate and other anion compounds in the environmental samples has been developed.
The method is based on the separation of nitrite, nitrate and sulfate from cation using anion exchange column for ion chromatograph, and detection of ammonia by indophenol reaction.
In the flow pathway of HPLC carrier, anion compounds are detected by conductivity detector, and postcolumn derivatization using spectrophotometric detector at 640 nm of wave length was determined ammonia.
An ammonia can be detected selectivity in water at 0.01 ppm concentration.
It was appllied determining of chloride, nitrite, nitrate, sulfate and ammonia in the environmental water samples such as lake, river, wastewater plant and rain by this method.
