DNA付加体の分析方法

—LC/ESI/MS/MS はポストラベルを越えたか？—

抄録

Quantification method of DNA adducts at the practical sensitivity was eagerly awaited in several study area such as mutagenesis, carcinogenesis, aging, DNA repair and molecular epidemiology. Recent development of liquid chromatography electrospray tandem mass spectrometry (LC/ESI/MS/MS) enables us to detect 25 fg of adducted deoxynucleoside, which is corresponding to a few DNA adducts in 10⁹ bases. This means that LC/ESI/MS/MS analysis of DNA adducts reaches to the practical sensitivity.