Journal of Ecotechnology Research
Online ISSN : 1884-0388
Print ISSN : 1881-9982
ISSN-L : 1881-9982
Original Article
Trial of Genotoxic evaluation for field-sampled microplastics by in vitro Micronuclei Test
FURUKUMA Shunji
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2020 Volume 19 Issue 2-3 Pages 9-15

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Abstract

A trial to genotoxic evaluate for field-sampled microplastics by in vitro micronuclei test using CHL/IU cells was conducted. Primary microplastics (plastic pre-production resin pellet mixtures) and secondary microplastics (product-derived plastic fragment mixtures) collected in the coast of Ainoshima Island, Kitakyushu City, Fukuoka Prefecture, Japan in 2017 and 2018 were examined. The collected samples were extracted at 0.2g/mL using the culture medium with FBS. In short treatment with S9 mix and continuous treatment without S9 mix, the micronuclei tests of these medium extract of plastic pre-production resin pellet mixtures were negative. On the other hand, the results of product-derived plastic fragment mixtures showed positive. This is the first study evaluated the in vitro genotoxicity of field-sampled plastic marine debris using an international standard test method.

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© 2018 International Association of Ecotechnology Research
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