Identification of the Gene Encoding VP7 of Serotype G3 Equine Rotavirus

Kenji TSUBOKURA; Hiroyuki HORIUCHI; Seiji SATO; Hiroki OSHIMA; Hiroshi IMAGAWA; Masanobu KAMADA; Haruo MATSUDA

doi:10.1294/jes.7.17

Notes

Identification of the Gene Encoding VP7 of Serotype G3 Equine Rotavirus

Kenji TSUBOKURA, Hiroyuki HORIUCHI, Seiji SATO, Hiroki OSHIMA, Hiroshi IMAGAWA, Masanobu KAMADA, Haruo MATSUDA

Author information

Kenji TSUBOKURA
Immunobiology Laboratory, Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739, Japan
Hiroyuki HORIUCHI
Immunobiology Laboratory, Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739, Japan
Seiji SATO
Immunobiology Laboratory, Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739, Japan
Hiroki OSHIMA
Immunobiology Laboratory, Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739, Japan
Hiroshi IMAGAWA
Epizootic Research Station, Equine Research Institute, Japan Racing Association, Tochigi 329-04, Japan.
Masanobu KAMADA
Epizootic Research Station, Equine Research Institute, Japan Racing Association, Tochigi 329-04, Japan.
Haruo MATSUDA
Immunobiology Laboratory, Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739, Japan

Keywords: equine, rotavirus, sequence, VP7

JOURNAL FREE ACCESS

1996 Volume 7 Issue 1 Pages 17-20

DOI https://doi.org/10.1294/jes.7.17

Details

Abstract

The sequence of the VP7-encoded gene of a serotype G3 equine rotavirus strain HO-5 isolated in Japan was determined. The VP7 was amplified by a reverse transcription-polymerase chain reaction from the segment 8 RNA of the HO-5 strain by using a synthetic primer pair for the rotavirus VP7 gene. The gene segment 8 of the HO-5 strain was also sequenced and the deduced amino acid sequence was determined. The results indicate that the VP7 of the HO-5 strain is encoded by the gene segment 8 and the deduced amino acid sequence of the HO-5 VP7 has an overall high sequence homology more than 95% to those of two known equine rotavirus strains.

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