2004 Volume 21 Issue 1 Pages 43-
An Amperometric flow injection system to measure the density of attached animal cells in hydroxyapatite-pulp composite fiber (HAPC) sheets was developed. 2,3,5-Trimethyl-1,4-benzoquinone (BQ) was injected into the HAPC sheet module containing animal cells as a mediator. 29375-Trimethyl-l,4-hydroquinone, which was produced by the reduction process of animal cells, was monitored amperometrically with a glassy carbon electrode. The carrier solution used was Dulbecco's phosphate buffered saline. A good linear relationship between the peak current and attached cell density was obtained in a range of 8.3×105 - 6.1×106 cells/sheet. The propose method can be applied for the measurement of cell density in a range of 8.3×105 - 1.6×107 cellslsheet. The peak current remained steady for 10 successive injections of 2,3,5-trimethyl-1,4-BQ at a concentration of 10 pM. Cytotoxicity due to 2,3,5-trimethyl-1,4-BQ was hardly observed at a concentration of less than 20 pM. This analytical system can be used for monitoring the cell density in bioreactors during the period in which the cells proliferate in the logarithmic phase.
