The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
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Simple detection of a yeast mitochondrial DNA-binding protein, Abf2p, on SDS-DNA gels
Isamu MiyakawaYoko KitamuraTakahiro JyozakiHiroshi SatoTakao Umezaki
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2000 Volume 46 Issue 6 Pages 311-316

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Abstract

Abf2p, a mitochondrial DNA-binding protein of yeast Saccharomyces cerevisiae, was selectively detected among mitochondrial nucleoid proteins by SDS-DNA polyacrylamide gel electrophoresis (SDS-DNA PAGE) followed by ethidium bromide staining. This method is simple and specific for the detection of Abf2p, and it may be used to identify an Abf2p-like protein that is present in mitochondrial nucleoids from other yeasts.

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© 2000 by The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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