2008 Volume 54 Issue 6 Pages 327-334
A new thermophilic spore-forming strain KG8T was isolated from the mud of Taslidere hot spring in Batman. Strain KG8T was aerobe, Gram-positive, rod-shaped, motile, occurring in pairs or filamentous. Growth was observed from 35-65ºC (optimum 55ºC) and at pH 5.5-9.5 (optimum pH 7.5). It was capable of utilizing starch, growth was observed until 3% NaCl (w/v) and it was positive for nitrate reduction. On the basis of 16S rRNA gene sequence similarity, strain KG8T was shown to be related most closely to Anoxybacillus species. Chemotaxonomic data (major isoprenoid quinone—menaquinone-7; major fatty acid—iso-C15:0 and iso-C17:0) supported the affiliation of strain KG8T to the genus Anoxybacillus. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain KG8T. Based on these results we propose assigning a novel subspecies of Anoxybacillus kamchatkensis, to be named Anoxybacillus kamchatkensis subsp. asaccharedens subsp. nov. with the type strain KG8T (DSM 18475T=CIP 109280T).