2011 Volume 57 Issue 6 Pages 341-346
A novel strain of Flavobacterium, DCY55T, a Gram-negative, yellow-pigmented, rod-shaped, non-spore-forming and gliding-motile bacterium, was isolated from the soil of a ginseng field in South Korea. Phylogenetic analysis, based on the 16S rRNA sequence, demonstrated that strain DCY55T belongs to the genus Flavobacterium within the family Flavobacteriaceae. Strain DCY55T showed the highest similarity with F. johnsoniae UW101T (97.1%), F. ginsenosidimutans THG 01T (96.8%), F. defluvii EMB 117T (96.6%), F. banpakuense 15F3T (96.3%) and F. anhuiense D3T (95.8%). Chemotaxonomic results showed that strain DCY55T predominantly contains menaquinone MK-6, that its DNA G+C content is 36.1mol%, and that its major cellular fatty acids are iso-C15:0, summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω 7c) and C16:0. The chemotaxonomic and genotypic characteristics support the taxonomic classification of strain DCY55T to the genus Flavobacterium. The results of physiological and biochemical tests confirmed that strain DCY55T is distinct from previously validated species. We conclude that strain DCY55T should be classified as a novel species of the genus Flavobacterium, for which the name Flavobacterium ginsengiterrae sp. nov. is proposed, with the type strain DCY55T (=KCTC 23319T = JCM 17337T).