2013 年 59 巻 1 号 p. 33-38
A novel actinobacterial strain, designated YM16-381T, was isolated from sediments of Lake Nakaumi in Shimane Prefecture, Japan. The cell of the strain was motile, non-spore-forming and Gram-positive. The colony was gray-pink and circular on marine agar 2216 medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belongs to the family Dermatophilaceae of the suborder Micrococcineae. The highest sequence similarity value of the isolate was 96.2% against Kineosphaera limosa. The diaminopimelic acid in the cell wall was meso-A2pm. The major menaquinone was MK-8(H4). The DNA G+C contents were 70.5 mol%. The major cellular fatty acids were C17:1ω8c, C16:0, C15:0 and C18:1ω9c. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and ninhydrin-positive phosphoglycolipid. On the basis of polyphasic taxonomic studies, strain YM16-381T represents a novel species of the genus Kineosphaera within the family Dermatophilaceae, for which the name Kineosphaera nakaumiensis sp. nov. is proposed. The type strain is YM16-381T (=KCTC 29138T=NBRC 109121T).