The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
Short Communications
Detection of active sorbitol-6-phosphate phosphatase in the haloacid dehalogenase-like hydrolase superfamily
Taejun ChinMasahiko Ikeuchi
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JOURNALS FREE ACCESS

2018 Volume 64 Issue 5 Pages 248-252

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Abstract

Sorbitol-6-phosphatase (EC 3.1.3.50) catalyzes sorbitol production from sorbitol-6-phosphate in certain organisms, but has not been identified unequivocally. We screened the activity of the haloacid dehalogenase-like hydrolases (HAD) superfamily and identified four HAD proteins from Escherichia coli as sorbitol-6-phosphatase. Of these proteins, HAD2 (YfbT) exhibited catalytic activity (kcat/Km) that was better than that of the previously reported "preferred" substrate. HAD1 (YniC) and HAD2 exhibited higher sorbitol-6-phosphatase activity than that of HAD12 (YbiV) and HAD13 (YidA). Therefore, genes of HAD may be useful for metabolic engineering of effective sorbitol production.

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© 2018, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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