The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
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Culturable fungal diversity and cellulase production by mixed culture Aspergillus fungi from Sanya mangrove
Siquan ZengJuan LingManzoor AhmadLiyun LinYanying ZhangCong WangJunde Dong
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2018 Volume 64 Issue 6 Pages 293-298

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Abstract

Fungi are the most suitable cellulase producers attributing to its ability to produce a complete cellulase system. 33 Genus, 175 Species fungi were isolated from Sanya mangrove, Hainan, China. Using congo red cellulose (CMC) medium, five fungi of cellulose-degrading were selected for further study. Molecular biology and morphological identification showed that all of these five fungi belong to Aspergillus fungi. The cellulase produced by these fungi were monitored during liquid state fermentation. The optimum conditions study for enzyme production illustrated that the highest activities appeared at pH 3.0, 35°C after fermentation for 3 days. Beyond that, the enzyme activity of mixed fungi is 11–26% higher than pure. The study demonstrated that mixed culture improved the hydrolysis of fungi cellulase.

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© 2018, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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