Functional diversification of five superoxide dismutase genes in Aspergillus nidulans against oxidative stress: distinct cellular roles of SodA and SodB

Abstract

Superoxide dismutases (SODs) play crucial roles in protecting cells against oxidative stress by catalyzing the dismutation of superoxide radicals. In Aspergillus nidulans, five putative SOD genes have been predicted in the genome; however, their comparative expression profiles and physiological functions remain largely uncharacterized. In this study, we analyzed the expression levels of all five SOD genes at different growth stages and examined the oxidative stress sensitivity of corresponding gene-disrupted strains. We found that sodA exhibited high and constitutive expression across all growth stages, while sodB was predominantly expressed in conidia (asexual spores). Disruption mutants of sodA and sodB showed increased sensitivity to oxidative agents, confirming their functional importance. Subcellular fractionation and SOD activity assays revealed that SodA was localized in the cytoplasm, whereas SodB was primarily localized in mitochondria. These results highlight the growth stage-specific expression and distinct cellular roles of SodA and SodB in A. nidulans, providing novel insights into the oxidative stress defense system in filamentous fungi.