Construction of transcription factor gene deletion library of Aspergillus luchuensis

Osamu Yamada; Naoko Nishibori; Risa Hayashi; Toshihide Arima; Osamu Mizutani

doi:10.2323/jgam.2020.09.001

This article has now been updated. Please use the final version.

Construction of transcription factor gene deletion library of Aspergillus luchuensis

Osamu Yamada, Naoko Nishibori, Risa Hayashi, Toshihide Arima, Osamu Mizutani

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Keywords: Aspergillus luchuensis, deletion library, transcription factor

JOURNAL FREE ACCESS Advance online publication
Supplementary material

Article ID: 2020.09.001

DOI https://doi.org/10.2323/jgam.2020.09.001

The final version of this article is now available: Vol. 67 (2021), No. 3 pp. 118-123

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Abstract

We selected 96 genes of Aspergillus luchuensis for the construction of a transcription factor gene deletion library. Of these, we successfully deleted 93 genes using Agrobacterium-mediated transformation (AMT) of A. luchuensis RIB 2604 ΔligD strains. We obtained only heterokaryonic strains after deletions of adaB, anBH1, hacA, hacB, hsf1, metR, and sonC gene, and additionally, could not obtain deletion strains for genes abaA and mcmA. The deletion strains will be available through our website (https://www.nrib.go.jp).

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