NUCLEIC ACID SYNTHESIS IN PSEUDOMONAS P

(1) EFFECTS OF CELL CONCENTRATION

Abstract

1. Logarithmically growing cells of Pseudomonas P were suspended to a final concentration of 25mg wet cells/ml ("conc. culture"). Under these conditions, synthesis of r-RNA is almost completely inhibited and m-RNA is detected, while syntheses of s-RNA and DNA continue.
2. When these cells are resuspended to a final concentration of 2mg wet cells/ml ("dil. culture"), synthesis of r-RNA begins. In "conc. culture 25min.-dil. culture" system, DNA, s-RNA and r-RNA syntheses occur at about the same rate, but in "conc. culture 100min.-dil. culture" system the specific synthesis of r-RNA is observed.