TRANSFORMATION IN BACILLUS SUBTILIS

I. AN APPARATUS FOR THE CONTINUOUS DEPROTEINIZATION OF NUCLEIC ACID SOLUTIONS

Abstract

An apparatus which allows the continuous deproteinization of nucleic acid solutions, reducing manual work, for methods in which the deproteinizing reagent is constituted by a liquid phase not mixing with the aqueous phase containing nucleic acids, is described. An example of the application on DNA from Bacillus subtilis is reported and the results regarding the extraction yield, percentage protein content, sedimentation coefficient, and the transforming activity are compared with those obtained by the original Marmur method.