The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
THE CULTIVATION OF RUMEN OLIGOTRICH PROTOZOA
I. FACTORS INFLUENCING THE LIFE OF ENTODINIA
TSUNEO HINDMASAO KAMETAKAMAKOTO KANDATSU
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1973 Volume 19 Issue 4 Pages 305-315

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Abstract

Various factors influencing the life of rumen protozoa, Entodinium species, were studied.
Optimal conditions for entodinial survival were determined for the following factors: Temperature, 38-40°; pH, 6.0-7.0; redox potential, -250 to -400mV; osmotic pressure, 0.7-1.0% NaCl equivalent. The influence of various compounds formed by rumen microorganisms was examined and toxic levels were found; ammonia had a harmful effect on the protozoa above the level of 3mmol/100ml; methylamine, histamine, and formate above 10mmol/100ml; methanol and ethanol above 30mmol/100ml. Acetate, propionate, butyrate, and lactate had no deleterious effect within the limits tested. In addition, the influence of antibiotics is described.

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