1982 Volume 28 Issue 5 Pages 409-416
The positions of double bonds in cellular monounsaturated fatty acids isolated from six representative strains of corynebacteria were determined by mass fragmentography. Hexadecenoic acid from Corynebacterium glutamicum CNF 016 and Brevibacterium ammoniagenes CNF 096 was principally the Δ7 isomer, while octadecenoic acid from these organisms was the Δ9 isomer. The monounsaturated fatty acids in Corynebacterium diphtheriae CNF 017 and Corynebacterium xerosis CNF 010 consisted mainly of the Δ9 isomers. In Corynebacterium equi CNF 002 and Corynebacterium fascians CNF 006, hexadecenoic acid was a mixture of Δ9 and Δ10 isomers, while octadecenoic acid was composed principally of the Δ9 isomer. The results suggest that the profiles of double bond location in unsaturated fatty acids are very useful for the taxonomic characterization of corynebacteria.