The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
CONSTRUCTION AND PHYSICAL MAP OF A BACILLUS SUBTILIS SPECIALIZED TRANSDUCING PHAGE ρ11 CONTAINING B. SUBTILIS LYS+ GENE
K. YAMANEY. TAKEICHIT. MASUDAF. KAWAMURAH. SAITO
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1982 Volume 28 Issue 5 Pages 417-428

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Abstract

The lys+ gene was detected as the smallest DNA. fragment (2.5×106 dalton) among the several genetic markers tested from EcoRI-cleaved B. subtilis chromosomal DNA preparations by agarose gel electrophoresis followed by identification with DNA mediated transformation experiments. The DNA fragment retaining the lys+ gene was cloned in a B. subtilis temperate phage ρ11 using the prophage transformation method. The molecular weight of the genome of the constructed specialized transducing phage (ρ11dlys+) was estimated to be 72-73×106. The phage was cross-reacted with an antiserum against ρ11 phage particles. The physical map of ρ11dlys+ DNA using SalI and BamHI was quite different from that of ρ11 DNA. ρ11dlys+ did not carry theρ11-coded thymidyrate synthetase gene.

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