SINGLE AND JOINT SEGREGATION OF MARKER LOCI IN THE SHIITAKE MUSHROOM, LENTINUS EDODES

Abstract

Single locus segregation was examined electrophoretically for six biochemical marker loci in single-spore-derived lines of Lentinus edodes. Only single banded electrophoretic patterns were observed, reflective of the hemizygous genetic constitution of these lines. Single locus segregation data for Aat-1 in one heterozygous line (131) was nonrandom by the chi-square test. Since only single spore recombinants for Aat-1 in this line were recovered and since another line (296) examined for Aat-1 segregated in Mendelian ratios, it is postulated that a locus carrying a lethal allele is tightly linked with Aat-1. One classical linkage was found (Gpi with Pgk, r=0.049) among 9 pairwise examinations for joint segregation.