ESTIMATION OF TIME LENGTHS OF CELL-CYCLE PHASES FROM ASYNCHRONOUS CULTURES OF THE BASIDIOMYCETOUS YEAST RHODOSPORIDIUM TORULOIDES

Abstract

Phase-lengths in the cell division cycle of the basidiomycetous yeast Rhodosporidium toruloides Banno in an asynchronous culture at 27° were estimated by using three parameters: cell morphology, DNA synthesis, and nuclear behavior. In the yeast-extract-sucrose medium, one division cycle is 160min, and phase-lengths of G1, S, G2, and M are calculated to be 116min, 27min, 10min, and 7min, respectively, by applying Powell's distribution function. As a dynamic phenomenon, the cell division cycle was presented in a cell clock.