The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
OCCURRENCE OF GLUTAMINE-2-OXOACID TRANSAMINASE ACTIVITY IN THE BLUE-GREEN ALGA ANABAENA CYLINDRICA
MASAYUKI OHMORITOSHIRO SAINOKAZUKO OHMORI
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1985 Volume 31 Issue 2 Pages 171-176

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Abstract

Aspartate and glutamate were formed from glutamine and oxaloacetate with crude enzyme extracts obtained by ammonium sulfate precipitation (35-65% saturation) from the blue-green alga Anabaena cylindrica. In this reaction the 2-amino group of glutamine was transferred to oxaloacetate in the absence of a reluctant. Azaserine, an inhibitor of glutamine -amide transfer, inhibited glutamate formation from glutamine but did not inhibit aspartate formation. Thus, a transamination from glutamine directly to oxaloacetate was indicated. This transamination reaction may be accomplished with glutamine-2-oxoacid transaminase which is found in animal tissues and in yeast.

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