The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
ISOLATION AND IDENTIFICATION OF CHOLESTEROL- DEGRADING RHODOCOCCUS STRAINS FROM FOOD OF ANIMAL ORIGIN AND THEIR CHOLESTEROL OXIDASE ACTIVITIES
KENJI WATANABEHISAKO SHIMIZUHIDETAKA AIHARARYO NAKAMURAKEN-ICHIRO SUZUKIKAZUO KOMAGATA
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1986 Volume 32 Issue 2 Pages 137-147

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Abstract

Sixteen bacterial strains that utilize cholesterol as sole carbon and energy sources were isolated from food of animal origins such as butter, bacon, pork fat, and chicken fat. Morphological, biochemical, physiological, and chemotaxonomic characteristics including cell wall chemotype, cellular fatty acid composition, DNA base composition, and DNA homologies revealed that these isolates belonged to the genus Rhodococcus Zopf. Most of them were identified as Rhodococcus erythropolis (Gray and Thornton) Goodfellow and Alderson and Rhodococcus equi (Magnusson) Goodfellow and Alderson. The cholesterol-degrading ability and extracellular and intracellular cholesterol oxidase activities of these strains were compared with those of related bacteria. Most of the authentic Rhodococcus strains degrade cholesterol. However, the amount of the enzyme varied among the strains, and some isolates produced large amounts of extracellular cholesterol oxidase.

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