1988 Volume 34 Issue 2 Pages 165-182
The presence of pyrophosphatidic acid (pyro-PA) was investigated in the lipid extracts of yeasts (241 strains belonging to 167 species of 43 genera). Pyro-PA can be clearly separated from other polar lipids by two-dimensional thin layer chromatography, and it can be distinguished from other phospholipids by its specific staining (purple blue) with Dittmer reagent. Pyro-PA was found exclusively in basidiomycetous species (127 strains belonging to 78 species of 14 genera). In contrast, no detectable amount of pyro-PA was found in the cellular lipids of ascomycetous species (114 strains belonging to 89 species of 31 genera). These results demonstrate that the determination of the presence of pyro-PA in the cellular lipids is useful in distinguishing ascomycetous from basidiomycetous yeasts.