MAPPING OF SPORULATION-DEFICIENT SUPPRESSOR MUTATIONS FOR A CATABOLITE-RESISTANT SPORULATION (crsA) MUTANT OF THE MAJOR SIGMA-43 FACTOR OF BACILLUS SUBTILIS

Abstract

Extragenic suppressor mutants for the sigA47 (crsA47, rpoD47) mutation were isolated and analyzed. Among 48 spontaneous suppressor mutants (sca), 13 were deficient in sporulation and these mutations were mapped in the spo0A, spo0B, and spo0F genes, respectively. During the mapping studies, spo0G14 and spo0D8 mutations were found to be alleles of the spo0A and spo0B genes, respectively. Thes results indicate that a link exists between the RNA polymerase sigA enzyme, glucose catabolite repression, and some of the spo0 gene functions.