EXPRESSION OF THE CHROMOSOME-CODED rRNA GENES OF PROTEUS VULGARIS IN ESCHERICHIA COLI

Abstract

To trace the fate of heterologous rRNA operons in vivo, a complete rrn operon from P. vulgaris was transferred into the chromosome of E. coli. This was done by phage λgt11 and by plasmid pOM40 which promotes the integration of the cloned insert into the malP locus. As derived from oligonucleotide analysis of 16S rRNA isolated from ribosomal 30S subunits the amount of heterologous 16S rRNA in the ribosomes of corresponding clones was determined to be about 5% of the homologous E. coil 16S rRNA.